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Do not combine with chelating solutions

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Your provider will walk you through proper technique, but general patterns include: Rotating injection sites reduces the likelihood of repeated irritation in one spot Mild redness or a small bump is common and usually resolves on its own Applying a clean, cool compress can ease temporary discomfort Proper reconstitution and injection technique reduce the chance of site reactions For step-by-step guidance on technique, see our complete injection guide

On the next day, after the membrane was washed with TBST for five times, HRP-conjugated secondary antibody (Thermo Scientific) was applied for 1.5 h at room temperature